Inhibition by Dithiothreitol of the Utilization of Glutamine by Carbamyl Phosphate Synthetase

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Inhibition by dithiothreitol of the utilization of glutamine by carbamyl phosphate synthetase. Evidence for formation of hydrogen peroxide.

The glutaminase activity of glutamine-dependent carbamyl phosphate synthetase (Escherichia coli) and that of the separated light subunit of this enzyme, as well as the glutaminedependent synthetase activity catalyzed by the native enzyme, are inactivated by incubation in air with relatively low concentrations (0.025 to 1 mM) of dithiothreitol; oxidized dithiothreitol does not inhibit. Other mer...

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ژورنال

عنوان ژورنال: Journal of Biological Chemistry

سال: 1974

ISSN: 0021-9258

DOI: 10.1016/s0021-9258(19)42872-x